Helicobacter pylori and EBV in gastric carcinomas: Methylation status and microsatellite instability

Table 1 Primer Sequences and PCR conditions for methylation-specific PCR (MSP) analysis

Gene	Primer (5’-3’) forward	Primer (5’-3’) reverse	Size (bp)	T (°C)	Ref.
COX2	M TTAGATACGGCGGCGGCGGC	TCTTTACCCGAACGCTTCCG	161	68	[24]
	U ATAGATTAGATATGGTGGTGGTGGT	CACAATCTTTACCCAAACACTTCCA	171	67
DAPK	M GGATAGTCGGATCGAGTTAACGTC	CCCTCCCAAACGCCGA	98	65	[25]
	U GGAGGATAGTTGGATTGAGTTAATGTT	CAAATCCCTCCCAAACACCAA	116	65
CDH1	M TTAGGTTAGAGGGTTATCGCGT	TAACTAAAAATTCACCTACCGAC	115	61	[26]
	U TAATTTTAGGTTAGAGGGTTATTGT	CACAACCAATCAACAACACA	97	59
hMLH1	M TATATCGTTCGTAGTATTCGTGT	TCCGACCCGAATAAACCCAA	153	65	[26]
	U TTTTGATGTAGATGTTTTATTAGGGTTGT	ACCACCTCATCATAACTACCCACA	124	63
CDKN2A	M TTATTAGAGGGTGGGGCGGATCGC	GACCCCGAACCGCGACCGTAA	150	70	[21]
	U TTATTAGAGGGTGGGGTGGATTGT	CAACCCCAAACCACAACCATAA	151	70