Proteomic analysis reveals molecular biological details in varioliform gastritis without Helicobacter pylori infection

Figure 3 Reverse transcription polymerase chain reaction analysis of thioredoxin domain-containing protein 5 and phosphatidylethanolamine-binding protein 1. A: Marked upregulation of thioredoxin domain-containing protein 5 (TXNDC5) in varioliform gastritis (V). The total RNAs of additional samples tissues were extracted by homogenization in Trizol (Invitrogen), cDNA synthesis was performed in 20 μL reaction system of reverse transcription including 5 μg RNA. Amplification of TXNDC5, with β2-MG acting as internal control, was carried out in DNA thermal cycler. PCR products were separated by 1.5% agarose gel electrophoresis. The bands were quantified by densitometry scanning. The relative quantification was calculated as the ratio of TXNDC5 expression to β2-MG expression as shown in the followed chart; B: Downregulation of phosphatidylethanolamine-binding protein 1 (PEBP1) in varioliform gastritis in comparison with that in normal (N) mucosa. The same experimental process was performed. The relative quantification was calculated as the ratio of PEBP1 expression to β2-MG expression as shown in the followed chart.