Bacteriolytic therapy of experimental pancreatic carcinoma

Figure 3 Quantitative analysis of cytotoxicity using flow cytometric carboxylfluorescein diacetate/propidium iodide staining. Lymphocytes were isolated from spleens and co-cultured with targets for five hours at E:T cell ratios of 10:1 and 30:1. Lymphocytes from successfully treated animals showed lytic activity against (A, B) syngeneic tumor cells with highest reactivity against CMT-93 cells at an E:T ratio of 30:1. However, cytotoxicity was found to be non-tumor specific, as reactivity was also observed against (C) non-malignant fibroblasts MC3T3-E1. Values are given as mean ± SE; ^aP < 0.05 vs tumor control; t-test.