Probiotic Bio-Three induces Th1 and anti-inflammatory effects in PBMC and dendritic cells

Figure 1 Effects of Bio-Three on peripheral blood mononuclear cells isolated from blood donors. Concentrations of interferon-γ (IFN-γ), interleukin (IL)-4, tumor necrosis factor-α (TNF-α), IL-10, and IL-12 p70 in the supernatants of peripheral blood mononuclear cells (PBMCs) (10⁵ cells/mL) incubated for 12 h were determined by enzyme-linked immunosorbent assay. The data shown are the values of different cytokines in the supernatants of PBMCs, which were collected at 1 and 2 wk after Bio-Three consumption, compared with controls. The results are presented as the mean ± SD.