Association of glypican-3 expression with growth signaling molecules in hepatocellular carcinoma

Figure 3 Down-expression of various molecules in glypican-3 siRNA-transfected hepatocellular carcinoma cell line. A: mRNA levels analyzed by real-time reverse transcription-polymerase chain reaction. The mRNA expressions of matrix metalloproteinase (MMP)2, MMP14, fibroblast growth factor receptor 1 (FGFR1), and insulin-like growth factor 1 receptor (IGF1R) were significantly down-regulated in HepG2 cells treated with glypican-3 (GPC3) specific siRNA (P < 0.05). Bar: SE; B: Protein levels analyzed by immunoblotting. The protein expressions of MMP2, MMP14, FGFR1, and IGF1R were significantly down-regulated in HepG2 cells treated with GPC3 specific siRNA; C: MMP2 activity analyzed by gelatin zymography. Conditioned media of siRNA-transfected HepG2 were used for gelatin zymography.