Hypoxia inducible factor-1α accumulation in steatotic liver preservation: Role of nitric oxide

Figure 2 Hypoxia-inducible factor-1α, nitrite/nitrate and endothelial nitric oxide synthase. A: Hypoxia-induced factor-1α (HIF-1α) protein levels quantified by Trans AM HIF-1α kit after 24 h of cold storage and 120 min of normothermic reperfusion; B: Representative Western blotting of HIF-1α (top) and densitometric analysis (bottom) after 24 h of cold storage and 120 min of normothermic reperfusion; C: Nitrite/nitrate levels after 120 min of normothermic reperfusion; D: Endothelial nitric oxide synthase (eNOS) protein levels in liver after 120 min of normothermic reperfusion. Representative Western blotting (top) and densitometric analysis (bottom). Cont 2: Liver flushed and perfused ex vivo without cold preservation; University Wisconsin (UW): Liver preserved in UW solution; IGL-1 + trimetazidine (TMZ) + NAME: Liver preserved in IGL-1 solution with TMZ and an nitric oxide synthesis inhibitor, L-NAME. ^aP < 0.05 vs Cont 2; ^cP < 0.05 vs UW; ^eP < 0.05 vs IGL-1; ^gP < 0.05 vs IGL-1 + TMZ.