Galangin induces apoptosis of hepatocellular carcinoma cells via the mitochondrial pathway

Figure 5 Effects of Bcl-2 on galangin-induced HepG2 cell apoptosis. A: Bcl-2 protein level was measured in HepG2 cells by Western blotting after cells were transfected; B: Morphology of apoptotic cells. Cells were treated with 185 μmol/L galangin for 24 h and stained with Hoechst 33258/PI to measure apoptosis. Pictures were taken under a 20 × objective. 1: Control vector group; 2: pcDNA3.1-bcl-2-transfected group; 3: Control siRNA group; 4: Bcl-2 siRNA-transfected group; C: Bcl-2 level affect apoptotic index of HepG2 cell induced by galangin. mean ± SD. n = 4. ^aP < 0.05 vs pcDNA3.1 group; ^cP < 0.05 vs control siRNA group; D: The effect of Bcl-2 level on cell viability induced by galangin. mean ± SD. n = 4. ^aP < 0.05 vs HepG2 cell/dimethyl sulfoxide-treatment group; ^cP < 0.05 vs pcDNA3.1-transfected group; ^eP < 0.05 vs control siRNA group.