Identification of cytokines involved in hepatic differentiation of mBM-MSCs under liver-injury conditions

Figure 5 ALB and urea production detection on day 20 after induction in a rescue experiment in which FGF-4, HGF and OSM were added back to the cytokine-removed medium treated with anti-FGF-4 (1:100), anti-HGF (1:100) and anti-OSM (1:100). A: Analysis of albumin secretion by ELISA; B: Urea production was measured using a colorimetric assay kit. MSC group: Control MSCs cultured in medium A with PBS; C group: MSCs induced with liver-injury conditioned medium only; E1-E4: MSCs induced with liver-injury conditioned medium including anti-FGF-4 (1:100), anti-HGF (1:100) and anti-OSM (1:100), as well as different concentrations of the cytokines (E1: 0 ng/mL FGF-4 + 0 ng/mL HGF + 0 ng/mL OSM; E2: 1.25 ng/mL FGF-4 + 2.5 ng/mL HGF + 1.25 ng/mL OSM; E3: 2.5 ng/mL FGF-4 + 5 ng/mL HGF + 2.5 ng/mL OSM; E4: 5 ng/mL FGF-4 + 10 ng/mL HGF + 5 ng/mL OSM). ^aP < 0.05, ^bP < 0.01 vs C group.