Identification of cytokines involved in hepatic differentiation of mBM-MSCs under liver-injury conditions

Figure 1 Analysis of albumin synthesis in cells for 20 d by ELISA (A_{450 nm}). MSC group: Control MSCs cultured in medium A with PBS; C group: MSCs induced with liver-injury conditioned medium with nonspecific IgG; (A) E1-E6 groups: MSCs induced with liver-injury conditioned medium and different combinations of specific antibodies (E1: Anti-FGF-3 + anti-FGF-4; E2: Anti-FGF-10 + anti-FGF-12 + anti-FGF13; E3: Anti-FGF-14 + anti-FGF-15 + anti-FGF-17; E4: Anti-FGF-18 + anti-FGF-20 + anti-FGF-21; E5: Anti-HGF + anti-OSM + anti-bNGF; E6: Anti-IGF-1 + anti-TGF-β1 + anti-TGF-β2 + anti-TGF-β3); (B) E1-E5 groups: MSCs induced with liver-injury conditioned medium and different specific antibody lonely (E1: Anti-FGF-4; E2: Anti-FGF-3; E3: Anti-bNGF; E4: Anti-HGF; E5: Anti-OSM). ^bP < 0.01 vs C group. ELISA: Enzyme-linked immunosorbent assay; MSC: Mesenchymal stem cells; PBS: Phosphate buffered saline; FGF: Fibroblast growth factor; HGF: Hepatocyte growth factor; OSM: Oncostatin M.