CD74 is a survival receptor on colon epithelial cells

Figure 1 CD74 is expressed on naive CEC isolated from C57BL/6 mice. CEC were isolated from C57BL/6, NOD/SCID and CD74-/- mice. A: RNA was purified and CD74 or HPRT mRNA were analyzed by RT-PCR. The results presented are representative of four separate experiments; B: Cells were lysed and proteins were analyzed by Western blotting; C: Cells were stained with anti cytokeratin and anti CD74 antibodies, and analyzed by FACS. Histograms show CD74 expression on cytokeratin positive cells. CEC: Colon epithelial cells; RT-PCR: Reverse transcriptase-polymerase chain reaction; FACS: Fluorescence-activated cell sorter.