Nardostachys jatamansi extract protects against cytokine-induced β-cell damage and streptozotocin-induced diabetes

Figure 4 NJE inhibits cytokine-induced activation of NF-κB and maintains glucose-stimulated insulin secretion in rat islets. Rat islets were treated with IL-1β (1 U/mL) and IFN-γ (100 U/mL) with or without 3 h pretreatment with NJE. Nitrite production and iNOS mRNA and protein expression (A) were determined after 24 h, and NF-κB DNA binding (B) was determined 1 h later; C: Rat islets (10 islets/500 μL) were treated with IL-1β (1 U/mL) and IFN-γ (100 U/mL) with or without 3 h pretreatment with NJE. Following 24 h incubation, glucose-stimulated insulin secretion was quantified. The results of triplicate samples are expressed as the mean ± SE. ^bP < 0.01 vs untreated controls; ^cP < 0.05, ^dP < 0.01 vs cytokine.