Identification of cytokines involved in hepatic differentiation of mBM-MSCs under liver-injury conditions

Table 1 Primers and annealing temperatures used for PCR

Gene	Sequence (5’-3’)	Annealing (°C)	Product (bp)
AFP-F	CACTGCTGCAACTCTTCGTA	52	300
AFP-R	CTTTGGACCCTCTTCTGTGA
HNF3b-F	GACCTCTTCCCTTTCTACCG	51	551
HNF3b-R	TTGAAGGCGTAATGGTGC
ALB-F	TCTTCGTCTCCGGCTCTG	55	475
ALB-R	CTGGCAACTTCATGCAAAT
TAT-F	CTTCAGTCCTGGATGTTCGC	55	619
TAT-R	CAGGGATTGGACGGGTTGTT
FGF4-F	CTGGTGGCTCACAGGACAATAAGAT	62	483
FGF4-R	GCTGGCTGAAGAAACAGGTAATAGGT
HGF-F	GTGCCAACAGGTGTATCAG	62	399
HGF-R	TGTCACAGACTTCGTAGCG
OSM-F	CTCACGGTCCACTACAACAC	62	123
OSM-R	GAGCCATCGTCCCATTCC
β-actin-F	TTCCTTCTTGGGTATGGAAT	55	200
β-actin-R	GAGCAATGATCTTGATCTTC

PCR: Polymerase chain reaction; AFP: α-fetoprotein; HNF: Hepatocyte nuclear factor; ALB: Albumin; TAT: Tyrosine aminotransferase; FGF-4: Fibroblast growth factor-4; HGF: Hepatocyte growth factor; OSM: Oncostatin M.