Comprehensive and innovative techniques for liver transplantation in rats: A surgical guide

Figure 6 Hepatic artery (HA) and biliary duct (BD) reconstruction. A: An initial suture is made through the whole layer of the CHA from the outside to the inside, and a thrusting is then performed through the whole layer of the PHA from the inside to the outside. Subsequently, reverse thrusting from the PHA to the CHA is performed with the same thread. The recipient’s PHA is then led into the graft CHA. One or two superficial stitches can be added if bleeding occurs; B: The diameter of the recipient’s PHA is approximately 0.2 mm. Complete ultra-microsurgery allows the use of end-to-end anastomosis in HA reconstruction; C: Intermittent and alternate clamping of the HA also achieves hemostasis; D: The previously ligated silk thread of the recipient’s BD is held using mosquito forceps. The recipient’s BD is encircled beforehand with silk thread. The biliary stent tube is led into the recipient’s BD. The recipient’s BD is open using the cut-down method, and the stent tube is inserted.