Fixation methods for electron microscopy of human and other liver

doi:10.3748/wjg.v16.i23.2851

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Jun 21, 2010 (publication date) through Dec 22, 2024

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Jun 21, 2010; 16(23): 2851-2866
Published online Jun 21, 2010. doi: 10.3748/wjg.v16.i23.2851

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Figure 15 A major problem in EM preparations of liver tissue is the occurrence of gaps in the endothelial lining. It is assumed that they appear during fixation due to instability of the cytoskeleton, which allows the fusion of fenestrae or the de novo formation of gaps. This SEM picture is from a rabbit liver fixed by portal perfusion.

Citation: Wisse E, Braet F, Duimel H, Vreuls C, Koek G, Olde Damink SW, van den Broek MA, De Geest B, Dejong CH, Tateno C, Frederik P. Fixation methods for electron microscopy of human and other liver. World J Gastroenterol 2010; 16(23): 2851-2866
URL: https://www.wjgnet.com/1007-9327/full/v16/i23/2851.htm
DOI: https://dx.doi.org/10.3748/wjg.v16.i23.2851