Fixation methods for electron microscopy of human and other liver

Figure 9 TEM micrograph of the tangentially cut endothelium of a human liver sinusoid, fixed by injection of glutaraldehyde fixative into a wedge biopsy. Underneath the thin layer of fenestrated endothelium, the Space of Disse shows the presence of microvilli, thin fibers of reticulin, and processes of fat-storing cells. Fenestrae in such preparations have an average diameter of about 105 nm. Original magnification 19 000 ×.