Fixation methods for electron microscopy of human and other liver

Figure 7 TEM micrograph of a transversely cut sinusoid of rabbit liver (A) and longitudinally cut sinusoid of mouse liver (B), fixed by perfusion through the portal vein. A: The Space of Disse shows the presence of microvilli extending from the parenchymal cells. Within the cytoplasm of the parenchymal cells, mitochondria, rough endoplasmic reticulum and glycogen are recognizable. Original magnification 6600 ×; B: Underneath the thin layer of fenestrated endothelium, bordering the sinusoidal lumen, the Space of Disse shows the presence of microvilli extending from the parenchymal cell surface. Within the cytoplasm of the parenchymal cells, mitochondria are recognizable. Original magnification 8900 ×.