Fixation methods for electron microscopy of human and other liver

Figure 6 Light micrograph of a wedge biopsy of human liver, injected with glutaraldehyde, plastic section stained with Toluidine Blue. A: Original magnification 10 × objective lens. Note that, as a result of successful perfusion, sinusoids are open, and only a few red blood cells are present. A portal tract is present in the upper right corner; B: Original magnification 63 × oil-immersion lens. Details such as giant mitochondria are visible in the parenchymal cells, amongst others such as small mitochondria, lipid inclusions and bile canaliculi. Sinusoids are patent and show the presence of sinusoidal cells.