Fixation methods for electron microscopy of human and other liver

Figure 5 Typical stub of aluminum (diameter 1 cm) bearing 11 pieces of liver tissue of an injection-fixed human liver wedge biopsy observed by SEM at low magnification. Strips of tissue in 100% ethanol are frozen at -196°C, fractured, critical point dried, sputter coated and glued to this carrier. The top surface of the specimens is observed, and imaging is achieved with secondary electrons. This picture is taken by a small CCD camera fitted to the microscope to observe specimens directly.