Protective effect of recombinant human IL-1Ra on CCl4-induced acute liver injury in mice

Figure 3 Immunostaining of proliferating cell nuclear antigen (PCNA) shows rhIL-1Ra promotes hepatocyte proliferation. A: Normal liver (original magnification, × 100); B: Normal liver in rhIL-1Ra treated mice (original magnification, × 100); C: Group received rhIL-1Ra at day 2 after CCl₄ administration, numerous PCNA⁺ hepatocytes in centrilobular areas and scattered PCNA⁺ hepatocytes at the edge of hepatocellular necrosis (original magnification, × 200); D: Group received PBS at day 2 after CCl₄ administration, few PCNA⁺ hepatocytes in centrilobular areas at day 2 (original magnification, × 200); E: Group received rhIL-1Ra at day 3 after CCl₄ administration, numerous positive cells in centrilobular areas around central vein (original magnification, × 100); F: Group received PBS at day 3 after CCl₄ administration shows fewer numbers of positive cells (original magnification, × 100); G: Numbers of PCNA⁺ cells after CCl₄ administration with rhIL-1Ra or PBS, at least 12 mm² tissue sections were counted for each mouse. Arrows point to PCNA+ hepatocytes. ^bP < 0.01.