Protective effect of recombinant human IL-1Ra on CCl4-induced acute liver injury in mice

Figure 2 Acute liver injury (1 mL/kg CCl₄ administration) ± rhIL-1Ra. A: Serum aspartate aminotransferase (AST); B: Serum alanine amino transferase (ALT), with rhIL-1Ra or PBS; C: Necrotic areas. Representative findings from at least 12 mm² tissue sections were counted for each mouse; D-F: Hematoxylin and eosin (HE) stained liver sections; D: Group received PBS at day 3 after CCl₄ administration, shows necrosis with clusters of inflammatory cells around central vein (original magnification, × 100); E: Group received rhIL-1Ra at day 3 after CCl₄ administration, demonstrates mostly histological recovery with only inconspicuous necrosis still remaining around central vein and very few inflammatory cells are present (original magnification, × 100); F: Normal liver histology with rhIL-1Ra treatment, which shows no difference from normal liver tissue histology. ^aP < 0.05, ^bP < 0.01.