Protective effect of recombinant human IL-1Ra on CCl4-induced acute liver injury in mice

Table 1 Injury grade

No. of mice	Day + 21	Day + 31	Day + 51	Day + 71
Group A (rhIL-1Ra)
1	III	II	I	0
2	III-IV	I	I	0
3	III	I	0	0
4	III	II	I	0
5	III-IV	I	0	I
6	IV	I	0	0
Group B (PBS)
1	III-IV	II-III	I-II	0
2	IV	III	II	I
3	III-IV	III	II	0
4	IV	II-III	II	0
5	IV	III	II	I
6	III-IV	III	I	I

¹Day of sacrifice after CCl₄ 1 mL/kg (1:3 dilution in corn oil) ip treatment. Injury grading with respect to severity of necrosis in liver parenchyma: Grade 0: Normal histology; Grade I: Presence of degenerated hepatocytes with only rare foci of necrosis; Grade II: Mild centrilobular necrosis around the central vein, occupying only a part of Rappaport’s zone III; Grade III: Established necrosis limited to zone III; Grade IV: Extensive, confluent centrilobular necrosis involving Rappaport’s zone III and II. rhIL-1Ra: Recombinant human interleukin 1 receptor antagonist.