Clinicopathological features and prognosis assessment of extranodal follicular dendritic cell sarcoma

Table 2 Primary antibodies and antigen retrieving procedures

Antibodies	Origins and clones	Dilutions	Antigen retrieval	Sources
Pan-cytokeratin	MAb, AE1/AE3	1:80	MW-citrate	Invitrogen
EMA	MAb, Mc5	1:100	MW-citrate	Invitrogen
Vimentin	MAb, V9	1:200	MW-citrate	NeoMarker
Desmin	MAb, ZC18	1:80	MW-citrate	Invitrogen
SMA	MAb, IA4	1:100	Not treated	Invitrogen
CD45	MAb, RP2/18	1:120	MW-citrate	Novacastro
CD68	MAb, KP1	1:200	MW-citrate	Zeta
S-100 protein	PAb, rabbit	1:400	MW-citrate	Dako
CD21	MAb, 2G9	1:20	AC-EDTA	NeoMarker
CD23	MAb, SP23	1:25	MW-citrate	NeoMarker
CD35	MAb, KuN241	1:20	AC-EDTA	NeoMarker
CD1a	MAb, MTB1	1:20	MW-citrate	Zeta
Podoplanin	MAb, D2-40	1:50	AC-EDTA	Zeta
p53 protein	MAb, DO-7	1:100	AC-EDTA	NeoMarker
Ki-67 antigen	MAb, MIB-1	1:200	AC-EDTA	Zymed

Antibody suppliers: Dako, Glostrup, Denmark; Invitrogen Corporation, Carlsbad, CA, USA; NeoMarkers Inc., Fremont, CA, USA; Novacastro Laboratories Ltd., Newcastle upon Tyne, UK; Zeta Corporation, Sierra Madre, CA; Zymed Laboratories, San Diego, CA, USA. EMA: Epithelial membrane antigen; MAb: Mouse monoclonal antibody; PAb: Polyclonal antibody; SMA: Smooth muscle-type actin; MW-citrate: Microwaving for 8 min in citrate buffer (pH 6.0); AC-EDTA: Autoclaving for 100 s in EDTA buffer (pH 9.0).