Hepatopoietin Cn suppresses apoptosis of human hepatocellular carcinoma cells by up-regulating myeloid cell leukemia-1

Figure 3 HPPCn inducing Mcl-1 expression via the MEK/ERK and SPK/S1P pathways. A: HPPCn induces Mcl-1 expression in a dose- and time-dependent manner and the densities of signals were determined by densitometry; B: Western blotting showing hybridization to anti-Mcl-1 antibody and anti-β-actin in HepG2 after the cells were treated with HPPCn for 3 h prior to pre-incubation with DMS, PD98059, LY294002, or AG490 for 1 h.