Treatment of biliary tract cancer with NVP-AEW541: Mechanisms of action and resistance

Figure 3 Cell cycle analysis. A: In vitro treatment of selected cell line EGI-1 with NVP-AEW541 for 36 h (n = 3); B: In vitro treatment of selected cell line Mz-ChA-1 (n = 3). Cells were stained with propidium iodide and analyzed by flow cytometry. ModFitLT 2.0 software was used to determine cell cycle proportions according to cellular DNA content; doublets were excluded by gating for width of fluorescence signal (FL2-W) (^aP < 0.05 for G0/G1 fraction, treated cells vs control); C: Cells having less than single DNA content (sub-G1 fraction) were presumed to be apoptotic (n = 3) (^aP < 0.05, treated cells vs control; error bars represent SD).