Apoptotic activity of caged xanthones from Garcinia hanburyi in cholangiocarcinoma cell lines

Figure 5 The four caged xanthones induce increase of Bax, AIF, activated caspase-3 and -9 while decreasing Bcl-2, survivin, procaspase-3 and -9 protein levels in CCA cell lines. KKU-100 and KKU-M156 cell lines were treated with DMSO or the four caged xanthones for the indicated times. Western blottings for each protein were performed as described in Materials and Methods. The mean ± SE of density reading of target protein normalized to β-actin was expressed in the respective box (n = 3). ^aP < 0.05, ^bP < 0.01 vs control. All four compounds showed the same result. The protein bands of the isomorellinol-treated cells were used as a representative.