Inhibition of hepatitis C virus replication by single-stranded RNA structural mimics

Figure 5 Western blotting analysis of NS3 protease gene expression in JFH-1 infected Huh7. 5 cells after transfection of 5B-74 mimic and controls. A: HCV NS3; B: Tubulin, 72 h after transfection. Lane 1: Levels prior to addition of mimic; Lane 2: HBV (unrelated) control; Lane 3: 5B-46 mimic; C: Quantification of Western blottings of NS3 protein in JFH-1-infected cells at baseline, after 72 h exposure to 5B-46, and to HBV unrelated control. Bound NS3 antibodies were detected with SuperSignal chemiluminescent substrate and quantitated by Gel Imaging software GeneTools.