Porcine hepatocyte isolation and reversible immortalization mediated by retroviral transfer and site-specific recombination

Figure 2 Isolation technique and microscopic appearance of primary and immortalized porcine hepatocytes. A: Perfusion was conducted by inserting a suitable pipette into vessels exposed on a cut surface of the sample; B: Non-immortalized primary porcine hepatocytes grow slowly with low plating efficiency and a short life span; C: Immortalized porcine hepatocytes grow rapidly as islands and had an extended life span. Magnifications: B and C 100 ×.