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World J Gastroenterol. Mar 28, 2010; 16(12): 1465-1472
Published online Mar 28, 2010. doi: 10.3748/wjg.v16.i12.1465
Published online Mar 28, 2010. doi: 10.3748/wjg.v16.i12.1465
Figure 3 Protein expression in Escherichia coli containing pET22b-PDGFRL.
Time course and different concentrations of isopropyl-b-D-thiogalactopyranoside (IPTG) analysis of pET22b-PDGFRL protein expression by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). M: Molecular weight standards; 1, 12: Uninduced bacterial lysate; 1-h (2-6), 2-h (7-11), 3-h (13-17), 4-h (18-22) induced samples at different concentrations of IPTG culture (0.1, 0.5, 1.0, 1.5, 2.0 μg/mL).
- Citation: Guo FJ, Zhang WJ, Li YL, Liu Y, Li YH, Huang J, Wang JJ, Xie PL, Li GC. Expression and functional characterization of platelet-derived growth factor receptor-like gene. World J Gastroenterol 2010; 16(12): 1465-1472
- URL: https://www.wjgnet.com/1007-9327/full/v16/i12/1465.htm
- DOI: https://dx.doi.org/10.3748/wjg.v16.i12.1465