Methylation of GATA-4 and GATA-5 and development of sporadic gastric carcinomas

Figure 3 Chromatogram of the methylated and unmethylated GATA-4 CpG islands by denatured high performance liquid chromatography (DHPLC). The 385-bp PCR product of the methylated and unmethylated GATA-4 were separated by DHPLC at a partial denaturing temperature 57.1°C and detected by a fluorescence (FL)-detector. The proportion of methylated GATA-4 in the tested samples was calculated according to the ratio of the peak area for the methylated GATA-4 to the total peak area for both the methylated and unmethylated GATA-4. The gray arrow points to the peak for the methylated GATA-4 (M) at the retention time 4.5 min; The black arrow points to the peak for the unmethylated GATA-4 (U) at the retention time 3.6 min. Genomic DNA of HCT116 was used as the GATA-4 methylation positive control. The inserted chart represents the dashed line surrounding the area. GATA-4 methylation was detected in the tested sample F0232.