Methylation of GATA-4 and GATA-5 and development of sporadic gastric carcinomas

Figure 2 Summary of MSP results of GATA-4 and GATA-5 methylation in SGCs (A), gastric dysplasia (B), and normal gastric mucosa samples (C). Each row represents a single sample. G4: GATA-4; G5: GATA-5; M in black, detection of methylated alleles; u in white, detection of unmethylated alleles only; ND in white, methylation information not available. T: Sporadic gastric carcinomas (SGC) tissue; N: SGC-paired corresponding normal tissue; S: Clinical stage (1-4) of SGCs; Hp: H. pylori infection positive (P in gray) or negative (N in light gray), detected with the 23S rRNA-PCR for the normal and corresponding normal gastric mucosa samples and with the ¹³C-urea breath test for subjects with gastric dysplasia. The GATA-4 methylation positive rate in low-grade GIN from patients with H. pylori infection was significantly higher than that from patients without infection (15/21 vs 1/7, P = 0.023). The GATA-5 methylation positive rate in low-grade GIN was significantly higher than that in indefinite for dysplasia (20/29 vs 35/75, P = 0.042). The GATA-4 and GATA-5 methylation rates in normal gastric biopsies were significantly lower than those in gastric dysplasia and SGC and the adjacent normal tissues (P < 0.001).