Establishment of a new quantitative detection approach to adefovir-resistant HBV and its clinical application

Table 2 Sensitivity of the new method for the detection of mixed variants

Variant: wild strain	HBV DNA reagent	rtA181 reagent	rtN236 reagent	a/b	a/c
0:1	6.06E+06	-	-
1:1	2.94E+06	4.80E+06	5.10E+06	0.61	0.58
1:10	3.98E+06	486 276.3	337 566.8	8.18	11.79
1:50	4.28E+06	83 242.16	85 867.71	51.42	49.84
1:200	5.63E+06	23  130.8	22 000	243.4	255.91
1:1000	5.76E+06	7880	6223.36	730.96	925.55
1:10 000	6.62E+06	-	-

a/b: The total DNA to rtA181 mutation-associated DNA; a/c: The total DNA to rtN236 mutation-associated DNA.