Loss of CD103+ intestinal dendritic cells during colonic inflammation

Figure 2 Inflammation induces infiltration of colonic LP and MLN with CD11c⁺ DC. Tissue was harvested from colonic LP (A/B) and MLN (C/D) of healthy animals (A/C) and mice with colitis (B/D). Immunohistochemistry was performed using an anti-CD11c⁺ antibody to stain for intestinal DC. Staining with isotype control revealed no background staining (data not shown). Representative sections from 5 mice per group are shown (magnification 100 ×). Staining was performed on tissue derived from colitic animals with transfer colitis as well as chronic DSS colitis and revealed similar results. Sections shown within this figure are derived from animals with transfer colitis.