Docosahexaenoic acid suppresses arachidonic acid-induced proliferation of LS-174T human colon carcinoma cells

Figure 2 Effect of DHA and AA on cell cycle and apoptosis. A: Cell cycle analysis by flow cytometry. Induction of apoptosis is indicated by an increased pre-G1 fraction. Results represent five independent experiments. B: DAPI staining of LS-174T cells incubated with different concentrations of AA and DHA showed a clear increase of apoptotic bodies in cells incubated with DHA. C: RT-PCR demonstrated induction of bcl-2 expression by AA, while DHA suppressed bcl-2 mRNA expression. ^aP < 0.01 versus control. n = 3 for each group. D: DHA induced transcription of p21, while AA did not alter p21 mRNA formation. ^aP < 0.01 versus control. n = 3 for each group.