Cell-permeable Tat-NBD peptide attenuates rat pancreatitis and acinus cell inflammation response

Figure 5 Effects of peptide at a dose of 10 mg/L in IL-1β mRNA expression in AR42J by RT-PCR. A: Images of agarose gel electrophoresis; 1: Control; 2: LPS; 3: Tat-NBD (WT); 4: NBD; 5: Tat-NBD (MT); 6: Tat; M: Marker; B: Tat-NBD (WT) peptide decreased IL-1β mRNA expression. Control: Cells were incubated with buffer control (n = 3); LPS: Cells were stimulated by LPS for 2 h (n = 3). Tat-NBD (WT): Pretreatment with 10 mg/L of (n = 3); NBD: Pretreatment with 10 mg/L of NBD (n = 3); Tat-NBD (MT): Pretreatment with 10 mg/L of Tat-NBD (MT) (n = 3); Tat: Pretreatment with 100 mg/L of Tat (n = 3). ^bP < 0.01, vs LPS group. ^dP < 0.01 vs LPS group.