High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells

Figure 3 Zymographic analysis of MMPs activity of tumor cells in DMEM with or without lymph node extract (A); the intensity of gelatin lysis bands obtained by scanning densitometry (LabWorks UVP GDS-800 Version 4. 0, multiple comparisons, ^aP < 0.05) (B). 1. L; 2. F; 3. P; 4. F(+); 5. F0; 6. F; 7. P; 8. F(+); 9. F0. 10. Type IV collagenases. 1. L: lymph node extract; 2-5: cells in DMEM. 6-10: cells cultured with lymph node extract. F: Hca-F cells; P: Hca-P cells; F(+): Hca-F cells transfected with pIRESpuro3 osteoglycin(+); F0: Hca-F cells transfected with pIRESpuro3.