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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Dec 14, 2009; 15(46): 5813-5820
Published online Dec 14, 2009. doi: 10.3748/wjg.15.5813
Published online Dec 14, 2009. doi: 10.3748/wjg.15.5813
Figure 1 Packaging system of LV-NT4(Si)-p53(N15)-Ant and LV-EGFP.
The packaging system: lentiviral vector, pLenti6/V5-D-TOPO, contains HIV-1 5'LTR, packaging signal (ψ), RRE sequence, CMV promoter and 3'LTR from which some regulatory sequences are deleted, resulting in “self-inactivation” of lentivirus after transduction of the target cells. pLP1, pLP2 and pLP/VSVG are three helper plasmids, which provide structural and replication proteins to produce lentivirus.
- Citation: Song LP, Li YP, Wang N, Li WW, Ren J, Qiu SD, Wang QY, Yang GX. NT4(Si)-p53(N15)-antennapedia induces cell death in a human hepatocellular carcinoma cell line. World J Gastroenterol 2009; 15(46): 5813-5820
- URL: https://www.wjgnet.com/1007-9327/full/v15/i46/5813.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.5813