Macrophage migration inhibitory factor regulates proliferation of gastric cancer cells via the PI3K/Akt pathway

Figure 2 Effects of rhMIF and LY294002 on cell cycle of MGC-803 cells. With or without 1-h pretreatment with 25 μmol/l LY294002, MGC-803 cells were exposed to 50 μg/L rhMIF for 24 h. A vehicle control of DMSO (less than 0.1%) was included. Cell lysates were prepared for the cell cycle detection. The cell cycle distribution was analyzed using an Eics-XL II flow cytometer. A: Control; B: MIF group; C: LY+MIF group; D: DMSO+MIF group; E: LY group; F: Quantification of G1/S viability(%) analysis of MGC-803 cells. Data were represented as mean ± SD of three independent experiments. ^aP < 0.05 vs control group; ^cP < 0.05 vs MIF group; ^eP < 0.05 vs LY+MIF group. DMSO (the menstruum of LY294002, less than 0.1%) had little impact on the rhMIF effect.