Immunotherapy for nonalcoholic steatohepatitis using the multiple cytokine production modulator Y-40138

Figure 3 Histological analysis of liver sections. A: Hematoxylin and eosin stain (× 100); B: Azan stain (× 100); C: Sirius red stain (× 100). Histological findings of liver tissue from the NASH group rats corresponded to Matteoni’s type 4 and grade 3/stage 3 of Brunt’s NASH classification. Sirius red staining revealed abundant collagen. NASH + Y-40138 group findings corresponded to Matteoni’s type 3 and Brunt’s grade 2/stage 2, and showed amelioration of inflammation and fibrosis in comparison to the NASH group (n = 5).