Brief Article
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Nov 28, 2009; 15(44): 5610-5619
Published online Nov 28, 2009. doi: 10.3748/wjg.15.5610
Table 1 The position, polymorphism and primer sequence in IL-2, IL-4 and IL-10 genes
SNP positionPolymorphismPrimer sequence 5’-3’PCR conditions
IL-2 -330T→GF: 5'-TATTCACATGTTCAGTGTAGTTCT-3'94°C, 48°C, 72°C for 1 min
R: 5'-ACATTAGCCCACACTTAGGT-3'
IL-4 -589C→TF: 5'-ACTAGGCCTCACCTGATACG-3'94°C, 57°C, 72°C for 30 s
R: 5'-GTTGTAATGCAGTCCTCCTG-3'
IL-10 -592A→CF: 5'-CCTAGGTCACAGTGACGTGG-3'94°C/30 s, 60°C/45 s, 72°C/60 s
R: 5'-GGTGAGCACTACCTGACTAGC-3'