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©2009 The WJG Press and Baishideng.
World J Gastroenterol. Nov 28, 2009; 15(44): 5610-5619
Published online Nov 28, 2009. doi: 10.3748/wjg.15.5610
Published online Nov 28, 2009. doi: 10.3748/wjg.15.5610
Table 1 The position, polymorphism and primer sequence in IL-2, IL-4 and IL-10 genes
SNP position | Polymorphism | Primer sequence 5’-3’ | PCR conditions |
IL-2 -330 | T→G | F: 5'-TATTCACATGTTCAGTGTAGTTCT-3' | 94°C, 48°C, 72°C for 1 min |
R: 5'-ACATTAGCCCACACTTAGGT-3' | |||
IL-4 -589 | C→T | F: 5'-ACTAGGCCTCACCTGATACG-3' | 94°C, 57°C, 72°C for 30 s |
R: 5'-GTTGTAATGCAGTCCTCCTG-3' | |||
IL-10 -592 | A→C | F: 5'-CCTAGGTCACAGTGACGTGG-3' | 94°C/30 s, 60°C/45 s, 72°C/60 s |
R: 5'-GGTGAGCACTACCTGACTAGC-3' |
- Citation: Gao QJ, Liu DW, Zhang SY, Jia M, Wang LM, Wu LH, Wang SY, Tong LX. Polymorphisms of some cytokines and chronic hepatitis B and C virus infection. World J Gastroenterol 2009; 15(44): 5610-5619
- URL: https://www.wjgnet.com/1007-9327/full/v15/i44/5610.htm
- DOI: https://dx.doi.org/10.3748/wjg.15.5610