Effects of angiopoietin-1 on attachment and metastasis of human gastric cancer cell line BGC-823

Figure 4 Expression of integrin β1 (A), CD44V6 (B), uPA (C) and MMP-2 (D) mRNA. 1: Markers: 100, 200, 300, 400, 500 and 600 bp; 2: Control group; 3: Ad-GFP-transfected group; 4: Ad-Ang-1-transfected group. A: Ang-1 group: 0.830 ± 0.010; control group and Ad-GFP group: 0.551 ± 0.009 and 0.543 ± 0.010, respectively; B: Ang-1 group: 0.570 ± 0.012; control group and Ad-GFP group: 0.451 ± 0.014 and 0.472 ± 0.013, respectively; C: Ang-1 group: 0.837 ± 0.081; control group and Ad-GFP group: 0.563 ± 0.059 and 0.506 ± 0.048, respectively; D: Ang-1 group: 0.351 ± 0.032; control group and Ad-GFP group: 0.153 ± 0.046 and 0.170 ± 0.023, respectively. These results show that the expression of integrin β1, CD44V6, uPA and MMP-2 mRNA was increased in the Ang-1 group (P < 0.05). Diagrams are representive of at least three independent experiments.