Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas

Figure 4 Immunofluorescent localization of WFS1 protein and vimentin in the pancreas of E18. 5, P0, P14, P21 and adult rats. The WFS1 antibody was detected with an FITC (green)-labeled secondary antibody and the vimentin antibody was detected with a Cy3 (red)-labeled secondary antibody on the same section. The overlap between AFP (green) and vimentin (red) labeling is colored orange. WFS1-positive cells can be detected in pancreas sections from E18.5 to adult. Double-labeling with antibodies to WFS1 and to vimentin revealed colocalization of these proteins in the same cells from E18.5 to adult. Primary objective magnification, × 400.