Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas

Figure 3 Immunofluorescent localization of WFS1 protein and glucagon in the pancreas of P0 and adult rats. The WFS1 antibody was detected with an FITC (green)-labeled secondary antibody and the glucagon antibody (mouse monoclonal antibody) was detected with a Cy3 (red)-labeled secondary antibody on the same section. The overlap between WFS1 (green) and glucagon (red) labeling is colored orange. WFS1-positive cells and glucagon-positive cells can be seen in 2 pancreas sections tested. Double-labeling with antibodies to WFS1 and glucagon reveal that the cells labeled with WFS1 are surrounded by α-cells. Primary objective magnification, × 400.