Expression and localization of Wolfram syndrome 1 gene in the developing rat pancreas

Figure 2 Immunofluorescent localization of WFS1 protein and insulin in the pancreas of E18. 5, P0, P14, P21 and adult rats. The WFS1 antibody was detected with an FITC (green)-labeled secondary antibody and the insulin antibody was detected with a Cy3 (red)-labeled secondary antibody. An overlap between WFS1 (green) and insulin (red) labeling is colored orange. Modest WFS1 staining is seen in the developing islets from E18.5 to adulthood, and overlaps insulin-positive β-cells. A few WFS1-labeled cells are scattered away from the islets. Primary objective magnification, × 400. Insets, higher magnifications of the areas indicated by arrows for E18.5, P0 and P14.