Effects on coagulation factor production following primary hepatomitogen-induced direct hyperplasia

Figure 3 Determination of plasma levels and activity for coagulation factors during the DH-mediated regenerative phase following TCPOBOP induction. Plasma activity levels of 10 coagulation factors (fibrinogen, prothrombin, factor V, VII, VIII, IX, X, XI, XII, and XIII) and plasma von Willebrand factor (VWF) antigen levels were assessed in mice during the DH-mediated regenerative phase. Activity levels of prothrombin, factor V, VII, VIII, IX, X, XI, and XII were measured by one-stage clotting assay, fibrinogen activities were measured by the Clauss method, factor XIII activities were measured by chromogenic assay, and VWF antigen levels were measured by specific ELISA. The plasma samples were obtained at D0, 3 h, D1, D2, D3, and D10 after TCPOBOP injection (n = 8/time point). The data were described as percentage of pooled normal plasma, and represented as the mean ± SE. ^aP < 0.05 vs D0.