Direct hepatic differentiation of mouse embryonic stem cells induced by valproic acid and cytokines

Figure 3 Gene expression analysis of VPA-induced hepatic lineage cells by RT-PCR and immunofluorescence staining. A: RT-PCR showed hepatic progenitor cells after the treatment with valproic acid expressing most typical markers of hepatic/hepatic stem cells; B: The hepatic progenitor-derived hepatocytes expressing typical markers of mature liver cells; C-E: Immunofluorescent images of AFP (C) and OCT-4 (D) staining in VPA-induced hepatic progenitor cells (E is the merged image of C and D); F-H: Immunofluorescent images of AFP (F) and CK19 (G) staining in VPA-induced hepatic progenitor cells (H is the merged image of F and G); I-K: Immunofluorescent images of AFP (I) and DLK (J) staining in VPA-induced hepatic progenitor cells (K is the merged image of I and J).