EP4 agonist alleviates indomethacin-induced gastric lesions and promotes chronic gastric ulcer healing

Figure 1 EP4 agonist effect on indomethacin-induced apoptosis in human gastric mucus epithelial cells (AGS). AGS cells were pre-treated with EP4 agonist for 30 min followed by addition of indomethacin for 24 h and sorted by flow cytometry after PI staining. Sub-2N population was quantified as apoptotic cells. A: indomethacin dose-dependently induced apoptosis, ^bP < 0.0001 vs the rest, n = 4; B: shown are values minus vehicle controls, ^aP < 0.005, n = 5-9; indomethacin dose was 400 μmol/L.