Costimulatory molecule programmed death-1 in the cytotoxic response during chronic hepatitis C

Figure 3 PD-L1 expression on Kupffer cells and PD-1 expression on HCV-specific CD8⁺ T cells. A: PD-L1-FITC FACS^® histograms gated on CD11b⁺ CD54⁺ cells from liver (L) and peripheral blood (PB), showing a higher PD-L1 expression on intrahepatic Kuppfer cells; B: FACS^® dot-plots and histograms of peripheral blood and intrahepatic T cells stained with CD8-Cy mAb, HCV-tetramers-PE and PD-1-FITC mAb. Gated Tetramer⁺/CD8⁺ cells are presented in the histograms for PD-1-FITC expression. PD-1 is up-regulated on intrahepatic HCV-specific CD8⁺ cells.