Expression of γ-synuclein in colorectal cancer tissues and its role on colorectal cancer cell line HCT116

Figure 4 Inhibition of cell migration and invasion by γ-synuclein knockdown. A: Boyden chambers with 8 μm polycarbonate membranes coated with or without 4 mg/mL growth factor-reduced Matrigel were used for migration or invasion assay. The chambers were stained with 0.2% crystal violet and analysed by photography, and the stained cells were migration or invasion cells in the lower chamber; B, C: The migration or invasion cells were counted in ten random fields of vision. Values were the number of cells relative to that of parental HCT116 cells, and expressed as mean ± SD for three independent experiments; ^aP < 0.05. Control: Parental HCT116 cells; pGCsi-vector: HCT116/vector cells; siRNA2: HCT116/siRNA2 cells.