Original Articles
Copyright ©2009 The WJG Press and Baishideng.
World J Gastroenterol. Jan 28, 2009; 15(4): 431-440
Published online Jan 28, 2009. doi: 10.3748/wjg.15.431
Table 1 Effect of stable expression of MDR1 targeting shRNA on MDR1 mRNA, P-gp and resistance to cytotoxic drugs in HepG2/mdr1 cells
Real-time PCR
P-gpDNR accumulationDrug resistance
mdr1 mRNAAdiramycinMytomycin
Cell line2-ΔΔCtMean fluorescenceMean fluorescenceIC50Fold-resistanceIC50Fold-resistance
HepG275.581.087.560.401.00.071.0
HepG2/m1.098.639.7625.062.59.67138.1
HepG2/m-sh56.30b1.07b79.32a0.56b1.4b0.08b1.14b