Characterization of CD133+ parenchymal cells in the liver: Histology and culture

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Oct 21, 2009 (publication date) through Feb 8, 2025

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World Journal of Gastroenterology

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1007-9327

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Baishideng Publishing Group Inc, 7041 Koll Center Parkway, Suite 160, Pleasanton, CA 94566, USA

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World J Gastroenterol. Oct 21, 2009; 15(39): 4896-4906
Published online Oct 21, 2009. doi: 10.3748/wjg.15.4896

Table 2 Sequences, annealing temperatures, cycle times, and product sizes of PCR primers

	F/R	Sequence	Temperature (°C)	Cycles	Size (bp)
CD133¹	1st F	GCCAGAAACTGTAATCTTAG	48	35	275
	1st R	TTACCTGGTGATTTGCCACA
	2nd F	CCTGGGGCTGCTGTTTATTA	55	35	153
	2nd R	ATCACCAACAGGGAGATTGC
CK19	F	TCCCGCGACTACAGCCACTACTACACGACC	55	35	745
	R	CGCGACTTGATGTCCATGAGCCGCTGGTA
CK7	F	GGATGCTGCCTACATGAGC	52	30	164
	R	CCAGGGAGCGACTGTTGT
AFP	F	GGGAGCGGCTGACATTATTA	50	30	231
	R	TCTTGCTTCATCGTTTGCAG
Albumin	F	TGCTTGAATGTGCTGATGACAGGG	50	30	161
	R	AAGGCAAGTCAGCAGGCATCTCATC
β-actin	F	CAAGAGATGGCCACGGCTGCT	55	30	334
	R	TCCTTCTGCATCCTGTCGGCA

¹Examined by nested PCR using first and second sets of primers. F: Forward; R: Reverse.

Citation: Yoshikawa S, Zen Y, Fujii T, Sato Y, Ohta T, Aoyagi Y, Nakanuma Y. Characterization of CD133⁺ parenchymal cells in the liver: Histology and culture. World J Gastroenterol 2009; 15(39): 4896-4906
URL: https://www.wjgnet.com/1007-9327/full/v15/i39/4896.htm
DOI: https://dx.doi.org/10.3748/wjg.15.4896